BUXORO VILOYATI SUV HAVZALARIDAN PROTOKKOK MIKROSUVO‘TLARINI (CHLOROPHYTA) AJRATISH, MORFOLOGIK IDENTIFIKATSIYA QILISH VA BIOTEXNOLOGIK KO‘PAYTIRISH
Keywords:
Chlorella vulgaris, microalgae isolation, organo-mineral medium, biomass productivity, Chlorophyta, Bukhara region, algologyAbstract
This study is devoted to the isolation, morphological identification, and biotechnological cultivation of microalgae belonging to the Protococcales group (division Chlorophyta) from three water bodies of the Bukhara region that differ fundamentally in their hydrochemical characteristics: the agricultural collectors Nometan and Kunji Qal’a, and the artificial water structure Labi Hovuz. Field samples were collected during spring (March–April) and summer (July–August) using an Apstein net (diameter 20 cm, mesh size No. 76). In the collected water samples, pH, NH4+, NO2-, NO3-, PO43-, SiO2, Cu, and Zn parameters were analyzed by colorimetric methods. Microscopic examination identified representatives of five genera: Chlorella, Scenedesmus, Ankistrodesmus, Pediastrum, and Chlorococcum; among them, Chlorella vulgaris Beijerinck and Scenedesmus obliquus showed clear dominance in all water bodies during summer. The isolated strains were cultivated in four nutrient media (Tamiya, Chu-13, Muzaffarov modification standard 04, and manure filtrate) under controlled conditions (temperature 25–30 C; photoperiod of 16 hours light / 8 hours dark). A new economically efficient organo-mineral medium (designated as 18.07) was developed and tested. By day 7, the highest cell density (35 million/ml) was recorded in standard 04 medium; the organo-mineral combination achieved 29 million/ml at considerably lower cost. The obtained results substantiate the possibility of economically efficient cultivation of high-biomass Chlorella vulgaris strains for aquaculture, livestock production, and phytoremediation purposes in countries with arid climates.
References
1. Adl SM, Bass D, Lane CE va boshqalar. Eukaryotlar klassifikatsiyasi, nomenklatirasi va xilma-xilligiga qayta ko‘rib chiqishlar. J Eukaryot Microbiol. 2019;66(1):4–119. https://doi.org/10.1111/jeu.12691
2. Menon KS, Harilal CC. CO2 boyitilgan va pH moslashtirilgan Bold Bazal muhitida Protococcus o‘sishini optimallashtirish. J Algal Biomass Util. 2016;7(3):245–258.
3. Dolganyuk V va boshqalar. Mikrosuvo‘tlar: qimmatli bioproduktlarning istiqbolli manbai. Biomolecules. 2020;10(8):1153. https://doi.org/10.3390/biom10081153
4. Manankina E, Melnikov S. Xlorelladan qishloq xo‘jaligi hayvonlarini oziqlantirish. V Mire Nauki. 2010;(8):5–7.
5. Allaguvatova R, Myasina Y, Zakharenko V, Gaysina L. Chlorella vulgaris Beijerinck suvo‘tlarini o‘stirishning oddiy usuli. IOP Conf Ser Earth Environ Sci. 2019;390:012020.
6. Chisti Y. Mikrosuvo‘tlardan biodiezel. Biotechnol Adv. 2007;25(3):294–306. https://doi.org/10.1016/j.biotechadv.2007.02.001
7. Ajala SO, Alexander ML, Olaoye OO, Ajayi AA. Oqova suvlarda mikrosuvo‘tlarning ozuqa moddalarini o‘zlashtirishi va biokimyoviy tarkibi. Environ Sci Pollut Res. 2020;27:13567–13580.
8. Mungunkhuyag B, Kim D, Lee J va boshqalar. Mikrosuvo‘tlar tomonidan og‘ir metallarning bioadsorpsiyasi: mexanizmlar va ekologik qo‘llanilish. Environ Pollut. 2022;292:118451.
9. Vazirzadeh A, Kazemi R, Mohammadi A. Chlorella vulgaris tomonidan fitoremedatsiya: nitrat, fosfat olib tashlash va biomassa sifati. Bioresour Technol Rep. 2022;18:123–136.
10. Borowitzka MA. Mikrosuvo‘tlardan yuqori qiymatli mahsulotlar: ularning rivojlanishi va tijoratlashtirish. J Appl Phycol. 2013;25(3):743–756. https://doi.org/10.1007/s10811 013-9983-9
11. Mata TM, Martins AA, Caetano NS. Mikrosuvo‘tlardan biodiezel ishlab chiqarish va boshqa qo‘llanishlar. Renew Sustain Energy Rev. 2010;14(1):217–232.
12. Komarek J, Fott B. Chlorophyceae (Yashil suvo‘tlar), Chlorococcales turkumi. Stuttgart: Gustav Fischer Verlag; 1983.
13. Bellinger EG, Sigee DC. Chuchuk suv suvo‘tlari: identifikatsiya va bioindikator sifatida qo‘llanilish. Chichester: Wiley-Blackwell; 2010.
14. Richmond A, Hu Q. Mikrosuvo‘tlar kulturasi qo‘llanmasi: amaliy fikologiya va biotexnologiya. 2-nashr. Oxford: Wiley-Blackwell; 2013.
15. Morando-Grijalva F va boshqalar. Chuchuk suv mikrosuvo‘tlar shtammlarining funksional tahlili va biotexnologik imkoniyatlari. Biotechnol Rep. 2020;28:e00538.
16. Ziganshina EE, Bulynina SS, Ziganshin AM. Chuchuk suv manbalaridan mikrosuvo‘tlar o‘sish kinetikasining qiyosiy tahlili. Front Microbiol. 2022;13:1–9.
17. Alharbi RM, Alzahrani A, Alghamdi A, Alasmari F. Yorug‘lik intensivligi va pH ning Chlorella vulgaris biomassasi va lipid ishlab chiqarishiga ta’siri. J Appl Phycol. 2024;36:1123–1135.
18. Becker EW. Mikrosuvo‘tlar: biotexnologiya va mikrobiologiya. Cambridge: Cambridge University Press; 1994.
19. Markou G, Nerantzis E. Yuqori qiymatli birikmalar va bioyoqilg‘i ishlab chiqarish uchun mikrosuvo‘tlar: stress sharoitlarida kultivatsiyaga e’tibor bilan sharh. Appl Energy. 2013;102:1–12.
20. Lee K va boshqalar. Yuqori lipid tarkibi uchun mikrosuvo‘tlarni ajratish va saralash. Int J Agric Biol Eng. 2014;7(1):58–64.
21. Ghani N va boshqalar. Kallar Kahar sho‘r ko‘lidan mahalliy mikrosuvo‘tlarni ajratish. Iran J Biotechnol. 2020;18(3):e2214.
22. Leelahakriengkrai P, Chaiyaso T, Techapun C. To‘rtta suv omboridan chuchuk suv mikrosuvo‘tlarining antioksidant faolligi va biokimyoviy tarkibi. Algal Res. 2021;56:102305.
23. Li F va boshqalar. Chlorella vulgaris yordamida oqova suvlarni tozalash va biomassa ishlab chiqarish. J Environ Manage. 2021;280:111678.
24. Yun HS va boshqalar. Chlorella vulgaris shtammlari uchun miksotrof va geterotrof kultivatsiya strategiyalari. Algal Res. 2021;59:102456.
25. Lortou U, Papadopoulou A, Kormas KA. 18S rRNK va ITS hududlari markerlari yordamida yashil mikrosuvo‘tlarni molekulyar identifikatsiya qilish. Phycologia. 2019;58(4):450–460.
26. Zhang Y va boshqalar. Selenga chidamli Chlorella vulgaris HNUFU001 ni ajratish, xarakterlash va fitoremedatsiya imkoniyatlari. Algal Res. 2023;72:103104.
