Aminokislotalarni titrametrik usulda taxlil qilish
Kalit so'zlar:
titrlash egri chizig'i, aminokislotalar, pH, biokimyo, pKa, izoelektrik nuqtaAnnotatsiya
Titirlash usuli yordamida noma’lum aminokislotani yoki aniq aminokislotaning noma’lum konsentratsiyasi aniqlanadi. Bu jarayonda tomchilatgan holda H+ yoki OH- ionlari berish orqali amalga oshiriladi. Titrlashni amalga oshirish uchun egri chiziqlari, pKa qiymatlari, bufer hududlarini aniqlash, protonlanish holatlari, zaryadlar va izoelektrik nuqtalarni bilish zarur. Bu ishda biz aminokislotalarni titirlashning nazariy asoslari va biokiyodan masalalar yechimiga to’xtalamiz.
Foydalanilgan adabiyotlar
1. Berg, J.M., Tymoczko, J.L., Stryer, L.: Biochemistry (2012) 7th edition, W. H. Freeman and Company, New York; ISBN-13: 9781429229364
2. Nelson, D.L., Cox, M.M.: Principles of Biochemistry (2008) 5th edition, W. H. Freeman and Company, New York; ISBN-13: 9780716771081
3. Voet, D., Voet, J.G., Prat, C.W.: Fundamentals of Biochemistry (2009) 3d edition, John Wiley et Sons, ISBN: 978-0-470-22842-5
4. Voet, D., Voet, J.G.,: Biochemistry (2011) 4th edition, John Wiley et Sons, ISBN-13: 978-0470570951
